14. AMBIO Nikon SoRa Spinning Disk Confocal CSU-W1
Description
Imaging Modes
- Spinning disk confocal (DAPI, CFP, GFP, YFP, mCherry, TxRED, Cy5)
- SoRa-mode (Super Resolution by Optical Pixel Reassignment, 1.4x resolution improvement)
- FRAP/ PA (photo activation/ conversion)
- Widefield EPI fluorescence mode (DAPI, GFP, TxRED, TRITC-B, Cy5)
- DIC for 10x, 40x, 60x, 100x objectives (upon request)
- Live-cell incubator (temperature, CO2, humidity)
7 Laser Lines
- 405 nm
- 445 nm
- 488 nm
- 515 nm
- 561 nm
- 594 nm
- 638 nm
Detectors
- 2x Hamamatsu ORCA-Fusion digital sCMOS cameras (2304x2304 pixels, 100 fps @ 2304x2048 pixels, 80% QE, 6.5 µm pixel size)
- Dual-camera system for simultaneous dual-color detection (e.g. GFP/RFP, RFP/SiR, GFP/SiR, upon request)
Software
- NIS-Elements AR (Advanced Research) including modules for:
- JOBS (automation)
- Deconvolution
- NIS.ai (Artificial Intelligence): denoise, clarify, enhance, segment, convert
Special
- Nipkow CSU-W for large FOV 1024x1024 px [size in µm for 10x]
- Uniform spinning disk illumination
- Enhanced signal-to-noise ratio compared to CSU-X
- 2 cameras for simultaneous dual-color imaging
- Fast emission filter wheel for clean multi-color imaging
- Ultra-fast laser switching for triggered multicolor acquisition
- SoRa-mode: Enhanced resolution (1.4x) in spinning disk mode
- Automatic water immersion dispenser (WID)
Emission Filters
| Filter | λ/Bandwidth |
| Quadband (DAPI, GFP, mCherry, Cy5) | 440/40 521/21 607/34 700/45 |
| DAPI | 447/60 |
| CFP | 472/30 |
| GFP | 525/50 |
| YFP | 542/27 |
| mCherry | 600/50 |
| Cy5 | 685/40 |
Objectives
| Mag. | Type | Immer. | NA | WD [μm] | Info |
| 10x (not installed) | Plan Achroat | Air | 0.45 | 4,000 | DIC N1 λ OFN25 |
| 40x | Apo LWD | Water | 1.15 | 600 | DIC N2 λS |
| 60x | Plan Apo | Water | 1.2 | 310-280 | DIC N2 VC OFN25 |
| 60x | Plan Apo | Oil | 1.4 | 130 | DIC N2 λ OFN25 |
| 25x | Plan Apo | Silicone | 1.05 | 550 | DIC N2 λS OFN25 |
| 40x | Plan Apo | Silicone | 1.25 | 300 | DIC N2 λS OFN25 |
| 100x | Plan Apo | Silicone | 1.35 | 300-290 | SR HP DIC N2 λS OFN22 |
Comments
New users - access upon training request here.