AMBIO Zeiss Upright LSM 900 Airyscan 2

Compact confocal for gentle multiplex (multi-channel, XYZT-dimensions, automation) imaging:

• 4 laser lines
• 2x GaAsP detectors, 1x Airyscan2 detector, 1x transmitted light detector
• Upright stage suited for fixed slides
• Monochromatic fluorescence camera
• Experiment-designer for optimal channel/ filter settings & acquisition speed
• Product page: https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html

Imaging modes

• Super-resolution & higher speed with Airyscan 2 (32x-array-detector for pixel re-assignment)
• Joint deconvolution of Airyscan 2 images for higher resolution
• Scanning confocal (DAPI, GFP, mCherry, Cy5, transmitted light)
• Improved confocal image quality with LSM Plus (linear Wiener filter for enhanced signal to noise & improved resolution)
• FRAP/PA (photo activation/conversion)
• Multiplex SR-2Y (increased speed with parallel scanning of 2 lines)

Lasers

• 385 nm
• 475 nm
• 555 nm
• 630 nm

Objectives

• 2.5x Plan-Apochromat (air), NA=0.085, working distance (WD)=8800 µm
• 10x Plan-Apochromat (air), NA=0.45, WD=2100 µm
• 20x Plan-Apochromat (air), NA=0.8, WD=550 µm
• 25x LD LCI Plan-Apochromat (multi immersion: water, silicone, glycerol, oil), NA=0.8, WD=570 µm, DIC 
• 40x LD LCI Plan-Apochromat (multi immersion: water, silicone, glycerol), NA=1.2, WD=410 µm, --> ONLY this objective is optimized for Airyscan mode! 
• 63x Plan-Apochromat (oil), NA=1.4, WD=190 µm, DIC