11. AMBIO Nikon Scanning Confocal A1Rsi+
Description
Imaging modes
- Scanning confocal (DAPI, GFP, mCherry, Cy5)
- FRAP/PA (photo activation/conversion)
- Live-cell incubator (temperature, CO2, humidity)
- Spectral scans
- FLIM/FCS
Lasers and max. intensities out of 60x oil objective
- 405 nm : 0.44 mW
- 488 nm : 0.80 mW
- 561 nm : 1.66 mW
- 647 nm : 1.70 mW
Emission Filters
Filter | λ/Bandwidth |
DAPI | 450/50 |
GFP | 525/50 |
mCherry | 595/50 |
Cy5 | 700/75 |
Scanner
- Point scanner (galvo)
- Resonant scanner (30 fps in 512x512 pixels, up to 420 fps in 512x32 pixels)
- Pinhole: hexagonal, tunable
Detectors
- 2x Hybrid GaAsP PMT (high sensitivity, GFP, mCherry)
- 2x PMT (Dapi, Cy5)
- Transmission light detector (DIC, phase)
- Spectral detector (optical diffraction grid and 32-channel multianodes)
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
10x | Plan Achroat | Air | 0.3 | 10,500 | |
20x | Plan Apo | Air | 0.8 | 1,000 | DIC N2 VC |
40x | Apo | Water | 1.25 | 180 | DIC N2 λ S |
60x | Plan Apo | Water | 1.27 | 170 | DIC N2 IR |
40x | Plan Flour | Oil | 1.3 | 200 | DIC N2 H |
60x | Plan Apo | Oil | 1.4 | 130 | λ |
Special
- Fast switch between galvo and resonant scanners
- Spectral scanner (unmixing)
Comments
New users - access upon training request here.