13. AMBIO Nikon Spinning Disk Confocal CSU-X
Description
Imaging modes
- Spinning disk confocal (DAPI, GFP, mCherry, Cy5)
- FRAP/ PA (photo activation/ conversion)
- Live-cell incubator (temperature, CO2, humidity)
Lasers and max. intensities out of 60x oil objective
- 405 nm : 3.54 mW
- 488 nm : 3.85 mW
- 561 nm : 4.11 mW
- 647 nm : 4.68 mW
Emission Filters
Filter | λ/Bandwidth |
Quad emission filter (DAPI, GFP, mCherry, Cy5) | 440/40 521/21 607/34 700/45 |
DAPI | 452/45 |
GFP | 525/50 |
mCherry | 600/50 |
Cy5 | 685/40 |
GFP narrow | 510/20 |
Detectors
- 2 EMCCD cameras (iXon3 DU-888 Ultra, 1024x1024 pixels, 13 μm pixel size, 26 fps full frame, usable area ~512x512 pixels, 93 fps)
- Current dual color dichroic for simultaneous GFP & mCherry detection
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
10x (not installed) | Plan Achroat | Air | 0.3 | 10,500 | |
20x | Plan Apo | Air | 0.8 | 1,000 | DIC N2 VC |
40x | Apo | Water | 1.25 | 180 | DIC N2 λ S |
60x | Plan Apo | Water | 1.27 | 170 | DIC N2 IR |
40x | Plan Flour | Oil | 1.3 | 200 | DIC N2 H |
60x | Plan Apo | Oil | 1.4 | 130 | λ |
100x | Plan Apo | Oil | 1.45 | 130 | λ |
Special
- Andor Revolution SD System (CSU-X)
- Uniform spinning disk illumination
- 2 cameras for ultra-fast dual color imaging
- Fast emission filter wheel for clean multi-color imaging of DAPI, GFP, mCherry, and Cy5
- Ultra-fast laser switching/ tuning (triggered acquisition)
Comments
New users - access upon training request here.