General

Systems is equipped for multiplexed 3D live-cell experiments:

• Multi-color, multi-position, time series, large image scan & automation
• Fully motorized high precision stage for accurate repositioning (XYZ-control)

• Stage incubator (temperature, CO2, humidity & carbogen)
• Perfusion
• Focus drift correction (Nikon PFS)
• Water immersion objectives for high-resolution on water-based samples

Imaging Modes

• Widefield epifluorescence (DAPI, CFP, GFP, YFP, mCherry & Cy5)
• Bright-field (transmitted light) for histological slides (color staining)
• Phase microscopy automated for 10x & 20x objectives
• Interference Reflection Microscopy (IRM)
• Polarized light microscopy
• FRET

LED Illumination (Lumencore, SpectraX) and max. intensities out of 40x objective

• 395 nm (395/25): 40 mW
• 440 nm (438/29): 69 mW
• 470 nm (475/28): 37 mW
• 508 nm (511/16): 8 mW
• 555 nm (555/28): 108 mW
• 640 nm (635/22): 26 mW

Emission Filters

Filter	λ/Bandwidth
Quad F67-401NXL (DAPI, GFP, mCherry, Cy5)	435/33 520/26 595/38 695/63
CFP ff01-482/35-32	482/35
YFP ET540/30, F47-543	540/30
GFP ET519/26M, F47-519NXL	519/26
mCherry BrightLight 595/31	595/31
Cy5 ET697/60M, F47-697NXL	697/60

Detectors

• For fluorescent samples: sCMOS, PCO.edge camera (>80% QE, 2048x2048 pixels, 6.5 µm pixel size, 53 fps full frame, up to 500 fps in small area)
• For stained samples (histology): Nikon DS-Ri2 color CMOS camera (4908x3264 pixels, 6 fps full frame, 45 fps in 1636x1088 pixels)

Objectives

Mag.	Type	Immer.	NA	WD [μm]	Info
4x	PlanApo λ	Air	0.2	20,000	OFN25, ∞/-
10x	PlanFluo Ph1 DL	Air	0.3	15,200	OFN25, ∞/1.2
20x	PlanApo Ph2 DM	Air	0.75	1,000	∞/0.17
40x	Plan Apo λ DIC N2	Air	0.95	250-170	OFN25, ∞/0.11-0.23
40x	Apo LWD λS DIC N2	Water	1.15	590-610	∞/0.15-0.19
60x	Plan Apo λ DIC N2	Oil	1.4	130	OFN25, ∞/0.17

Special

• Fast LED switching (triggered acquisition)
• Fast emission filter wheel for clean multi-color imaging of DAPI, CFP, GFP, YFP, mCherry & Cy5
• Automatic water immersion dispenser (WID) for water immersion objectives
• NIS-Elements AR (Advanced Research) software including modules for: JOBS (automation), 3D Deconvolution & NIS.ai (artificial intelligence): denoise, clarify, enhance, segment & convert