17. AMBIO Nikon Multiphoton Confocal
Description
Imaging modes
- Multiphoton confocal
- DIC, relief contrast
Laser (Mai Tai Insight Deepsee Dual)
- Tunable: 680 nm (600 mW) – 900 nm (500 mW) – 1300 nm (500 mW)
- Single line: 1040 nm
Emission Filters
Filter | λ/Bandwidth |
DAPI | - |
GFP | 525/50 |
mCherry | 595/50 |
Cy5 | 700/75 |
Scanner
- Point scanner (galvo)
- Resonant scanner (30 fps in 512x512 pixels, up to 420 fps in 512x32 pixels)
Non-Descanned Detectors (no pinhole)
- 2x GaAsP-NDD (highly sensitive, GFP, mCherry)
- 2x PMT-NDD (DAPI, Cy5)
Objectives
Mag. | Type | Immer. | NA | WD [μm] | Info |
25x | Apo | Water | 1.1 | 2,000 | NCC |
40x | Apo | Water | 0.8 | 3,500 | NIR |
Special
- Upright
- Extended IR laser for deep tissue imaging
- Fast switch between glavo and resonant scanner
Comments
New users - access upon training request here.