Imaging Mass Cytometry (IMC) is an approach for the targeted and epitope-specific multiparameter analysis of tissue sections (Scheme). In contrast to conventional immunohistochemistry or immunofluorescence, this technology allows for the simultaneous detection of >35 specific markers on the tissue by using metal-isotopes and their different masses as analytes. Tissue sections are labeled with epitope-specific, metal isotope conjugated antibodies and the tissue is ablated in 1x1 µm spots by a laser. The ablated material is carried into the mass cytometer, and the metal ion content of each pixel is analyzed by time-of-flight mass spectrometry. The signals are then plotted as a multiparameter image that can be analyzed by any imaging software. For more information about this new technology contact the core facility.

Measurement of tissue samples

IMC is offered as service (staff-operated) or independent usage with assistance. A project meeting is required to get access. We will discuss your experimental setup, technical details and the timeline for your sample processing. Please request a project meeting as early as possible, but latest 6 weeks before onset of your experiment.

Please download the project registration form here and attach it to the request.

Register for overhead funding with the BIH (services -> provider Berlin Institute of Health)